Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0016cpr2 | Development of The Follicleand Corpus Luteum | CPR2001

Apoptosis during folliculogenesis in pigs

Guthrie H. D. , Garrett W. M.

The number of female germ cells in pig fetuses decreases by 70% between day 50 after mating and day 300 after birth. Approximately 55% of antral follicles undergo degeneration (atresia) except during the 3 days before oestrus, when only 15% of the follicles survive to ovulate. Apoptosis, a form of programmed cell death, is recognized as the mechanism of germ cell death and follicle atresia at all stages of folliculogenesis. The internucleosomal cleavage of genomic DNA ca...

bp0016cpr11 | Fertilization in vivo and in vitro | CPR2001

Gamete adhesion molecules

Millerand D. J. , Burkin H. R.

Despite the importance of fertilization for animal production, species preservation and controlling reproduction, the molecular basis underlying fertilization is not well understood. More progress has been made in mice than in other mammals, but targeted deletion of specific genes in the mouse has often yielded unexpected results. The pig is also a useful animal to study, as large numbers of pig gametes can be acquired easily. However, it appears that the pig zona pelluc...

bp0006rdr14 | Reproductive Manangement | REDR2006

Technologies for fixed-time artificial insemination and their influence on reproductive performance of Bos indicus cattle

Bó GA , Cutaia L , Peres LC , Pindnato D , Maraña D , Baruselli PS

The adaptation of Bos indicus cattle to tropical and subtropical environments has led to their widespread distribution around the world. Although artificial insemination (AI) is one of the best alternatives to introduce new genetics into Bos indicus herds, the peculiarity of their temperament and the tendency to show short oestrus (many of them during the night) greatly affects the effectiveness of genetic improvement programs. Therefore, the most useful alte...

bp0015cpr13 | Boar Fertility and Semen Preservation | CPR1997

Physiology of the Meishan boar

Lunstral D. D. , Ford J. J. , Klindt J. , Wise T. H.

Onset of puberty (sperm production) occurs at a much younger age (56-84 days) in Meishans than in conventional boars (120-180 days). Throughout postnatal development, Meishans exhibit markedly higher (two- to ten-fold) concentrations of serurn FSH, LH and androgens compared with conventional boars, and these high hormone concentrations are maintained at maturity. Increased gonadotrophin concentrations occur only in Meishan males, since concentrations in female Meishans a...

bp0016cpr20 | New Technologies | CPR2001

Applying functional genomics research to the study of pig reproduction

Pomp D. , Caetano A. R. , Bertani A. R. , Gladney C. D. , Johnson R. K.

Functional genomics is an experimental approach that incorporates genomewide or system-wide experimentation, expanding the scope of biological investigation from studying single genes to studying potentially all genes at once in a systematic manner. This technology is highly appealing because of its high throughput and relatively low cost. Furthermore, analysis of gene expression using microarrays is likely to be more biologically relevant than the conventional paradigm ...

bp0018cpr4 | Physiological Roles of The Boar Ejaculate | CPR2009

Recent advances in boar semen cryopreservation

Rath D. , Bathgate R. , Rodriguez-Martinez H. , Roca J. , Strzezek J. , Waberski D.

Since 35 years ago boar semen has been frozen and used for artificial insemination (Al). However, fertility of cryopreserved porcine sperm has consistently been low as boar sperm are more sensitive to cellular stress imposed by changing osmotic balance, oxidative stress, low-temperature exposure, cryo-protectant intoxication etc. and are less able to compensate for these deficiencies at commercially applicable dosages. Additionally, differences in sperm freezability amon...

bp0001redr10 | (1) | REDR1980

Endocrine patterns associated with puberty in male and female cattle

Schams D , Schallenberger E , Gombe S , Karg H

Summary. In four studies secretion patterns of LH, FSH, prolactin, testosterone and progesterone were measured in male and female cattle to determine endocrine changes associated with sexual maturation. Two periods of increasing gonadotrophin secretion were observed, the second one coinciding with puberty. A short luteal phase of 8–12 days precedes the first oestrus at 10–11 or 14 months of age. The testosterone values of the bulls increased with age...

bp0007rdr22 | The Ruminant Corpus Luteum | REDR2010

Regulation of corpus luteum development and maintenance: specific roles of angiogenesis and action of prostaglandin F

Miyamoto A , Shirasuna K , Shimizu T , Bollwein H , Schams D

Development of the corpus luteum (CL) in ruminants occurs in a rapid and time-dependent manner within 1 week after ovulation, with morphologic and biochemical changes in the cells of the theca interna and granulosa cells of the preovulatory follicle. These changes involve luteinisation of steroidogenic cells and angiogenesis to establish normal luteal function (progesterone secretion). The CL is composed of a large number of vascular endothelial cells, large and small steroido...