Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0016cpr22 | New Technologies | CPR2001

Deep intrauterine insemination and embryo transfer in pigs

Martinez E. A. , Vazquez J. M , Roca J. , Lucas X. , Gil M. A. , Vazquez J. L.

A new method for non-surgical deep intrauterine catheterization of pigs, without sedation of the sow, is described. Insemination results obtained with this method using fresh spermatozoa demonstrate that, in comparison to conventional artificial insemination (Al) (3 × 109 spermatozoa in 80-100 ml), a 20-60-fold reduction in the number of spermatozoa inseminated and at least a 8-10-fold reduction in the dose volume can be used without affecting fertility ...

bp0017cpr19 | (1) | CPR2005

Strategies to improve the fertility of frozen-thawed boar semen for artificial insemination

Roca J. , Rodriguez-Martinez H. , Vazquez J.M. , Bolarin A. , Hernandez M. , Saravia F. , Wallgren M. , Martinez E.A.

Although cryopreservation of boar semen for artificial insemination (Al) was developed 35 years ago, cryopreservation conditions and Al strategies are still considered sub-optimal. Al with excessive numbers of frozenthawed sperm (5-6 x 109 cells), still does not achieve fertility levels similar to Al using liquid semen because of reduced sperm survival. Frozenthawed (FT) spermatozoa have therefore not been the preferred option for commercial breeding programme...

bp0018cpr3 | Physiological Roles of The Boar Ejaculate | CPR2009

Optimal characteristics of spermatozoa for semen technologies in pigs

Parrilla I. , Vazquez J.M. , Caballero I. , Gil M.A. , Hernandez M , Roca J. , Lucas X. , Martinez E.A.

Despite the great potential of sperm technologies such as sperm cryopreservation and sperm sex sorting for the improvement of different aspects of swine production, artificial insemination with fresh or stored semen is currently the only sperm technology used at a commercial scale in the pig industry. The lower reproductive performance associated with the use of these sperm technologies is the reason for such limited use. Since optimal characteristics are required for su...

bp0018cpr4 | Physiological Roles of The Boar Ejaculate | CPR2009

Recent advances in boar semen cryopreservation

Rath D. , Bathgate R. , Rodriguez-Martinez H. , Roca J. , Strzezek J. , Waberski D.

Since 35 years ago boar semen has been frozen and used for artificial insemination (Al). However, fertility of cryopreserved porcine sperm has consistently been low as boar sperm are more sensitive to cellular stress imposed by changing osmotic balance, oxidative stress, low-temperature exposure, cryo-protectant intoxication etc. and are less able to compensate for these deficiencies at commercially applicable dosages. Additionally, differences in sperm freezability amon...

bp0018cpr1 | Physiological Roles of The Boar Ejaculate | CPR2009

The physiological roles of the boar ejaculate

Rodriguez-Martinez H. , Kvist U. , Saravia F. , Wallgren M. , Johannissono A. , Sanz L. , Pena F.J. , Martinez E.A. , Roca J. , Vazquez J.M. , Calvete J.J.

During ejaculation in the boar, sperm cohorts emitted in epididymal cauda fluid are sequentially exposed and resuspended in different mixtures of accessory sex gland secretion. This paper reviews the relevance of such unevenly composed fractions of seminal plasma (SP) in vivo on sperm transport and sperm function and how this knowledge could benefit boar semen processing for artificial insemination (Al). The firstly ejaculated spermatozoa (first 10 ml of the spe...