Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0016cpr13 | Embryonic and Placental Development | CPR2001

Gene expression during pre- and peri-implantation embryonic development in pigs

Maddox-Hyttel P. , Dinnyes A. , Laurincik J. , Rath D. , Niemann H. , Rosenkranz H. , Wilmut H.

Embryo technological procedures such as in vitro production and cloning by nuclear transfer are not as advanced in pigs as in cattle and cannot yet be applied under field conditions. The present paper focuses on genome activation in in vivo-derived, in vitro-produced and nuclear transfer pig embryos with special emphasis on the development of embryonic nucleoli, where the ribosomal RNA (rRNA) genes transcribed can be used as markers for genome ...

bp0018cpr4 | Physiological Roles of The Boar Ejaculate | CPR2009

Recent advances in boar semen cryopreservation

Rath D. , Bathgate R. , Rodriguez-Martinez H. , Roca J. , Strzezek J. , Waberski D.

Since 35 years ago boar semen has been frozen and used for artificial insemination (Al). However, fertility of cryopreserved porcine sperm has consistently been low as boar sperm are more sensitive to cellular stress imposed by changing osmotic balance, oxidative stress, low-temperature exposure, cryo-protectant intoxication etc. and are less able to compensate for these deficiencies at commercially applicable dosages. Additionally, differences in sperm freezability amon...

bp0017cpr23 | (1) | CPR2005

Structural, biochemical and functional aspects of sperm-oocyte interactions in pigs

Rath D. , Topfer-Petersen E. , Michelmann H. W. , Schwartz P. , von Witzendorff D. , Ebeling S. , Ekhlasi-Hundrieser M. , Piehler E. , Petrunkina A. , Romar R.

Polyspermic fertilization is still a major issue in porcine IVF systems. New information is available to characterize the zona pellucida (ZP) at different developmental stages by scanning electron microscopy (SEM) and by confocal microscopy to show the distribution of ZP glycoproteins. SEM images indicated no differences between in vivo and in vitro matured oocytes; however a change in the surface structure between immature and matured oocytes, as well as between mature ...