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Previous issue | Volume 10 | ISED2019

3rd International Symposium on Embryonic Diapause

bp0010ised2 | (1) | ISED2019

A role for Msx genes in mammalian embryonic diapause

Cha J , Fenelon JC , Murphy BD , Shaw G , Renfree MB , Dey SK

Mammalian embryonic diapause is a reproductive phenomenon defined by the reversible arrest in blastocyst development and metabolic activity within the uterus which synchronously becomes quiescent to implantation. This natural strategy, evident in over 130 species across eight orders, can temporally uncouple conception from delivery until conditions are favorable for the survival of the mother and newborn. While the maternal endocrine milieu has been shown to be important for t...

bp0010ised3 | (1) | ISED2019

Conserved mechanisms for putting pregnancy on hold in the mouse, mink and tammar wallaby

Fenelon JC , Shaw G , Renfree MB , Murphy BD

Embryonic diapause was first identified over 150 years ago, but many questions still remain about how the external and hormonal controls of embryonic diapause translate into how the uterus conveys information to the embryo. Current evidence suggests that the control of diapause is mediated by components of the uterine secretions. However, the identity of the essential signalling molecule(s) is unknown. The mouse (Mus musculus), the mink (Neovison vison) and t...

bp0010ised4 | (1) | ISED2019

Embryonic diapause in the European roe deer (Capreolus capreolus)

van der Weijden VA , Ulbrich SE

Keywords: Embryonic diapause, European roe deer, embryo-maternal communication© Third International Symposium on Embryonic Diapause...

bp0010ised5 | (1) | ISED2019

Could embryonic diapause facilitate conservation of endangered species?

Wauters J , Jewgenow K , Goritz F , Hildebrandt TB

During embryonic diapause, the development of the embryo is paused shortly after conception by metabolic or seasonal factors. When conditions become favorable, the embryo will resume development after reactivation by the maternal reproductive system. Inducing this resting state in in vivo or in vitro manipulated embryos may offer invaluable advantages in the long-term storage of embryos, therefore offering a potential worthwhile and novel alternative in assisted reproduction c...

bp0010ised6 | (1) | ISED2019

Models of embryonic diapause in Carnivora

Murphy BD , Fenelon JC

The phenomenon of obligate embryonic diapause, comprising developmental arrest at the blastocyst stage, has been recognized to occur in more than 60 species in three families in the Order Carnivora. The evolutionary advantage believed to be conferred by this trait is that it permits mating and parturition to occur at the most favorable times of year for reproductive success and offspring survival. The carnivore blastocyst in diapause consists of hundreds of cells, configured i...

bp0010ised7 | (1) | ISED2019

Identification of mink (Neovison vison) fecal proteins during embryonic diapause and placental pregnancy for non-invasive pregnancy diagnosis in wildlife

Curry E , Easley JS , Wojtusik J , Roth TL

Currently, there is no method to diagnose pregnancy non-invasively in most wildlife species that experience delayed implantation and pseudopregnancy, either during embryonic diapause or placental pregnancy. The aim of this study was to utilize farm-raised mink (Neovison vison) as a model species to evaluate changes in the fecal proteome associated with pregnancy. Specific objectives were to: 1) determine if fecal peptides were differentially abundant in parturient ver...

bp0010ised8 | (1) | ISED2019

Embryonic diapause in the short-tailed fruit bat, Carollia perspicillata: why this is postimplantational

Rasweiler JJ , Badwaik NK

Pregnancy has been studied in Carollia perspicillata bred in captivity or collected from a reproductively-synchronized wild population on Trinidad, West Indies. In both situations, periods of postimplantational embryonic diapause were sometimes observed. In captivity, this was induced by stress, or once-weekly periods of food deprivation, and resulted in gestation periods of highly variable duration (105-237 days). The normal gestation period for this species is 113-1...

bp0010ised9 | (1) | ISED2019

Lipid droplet utilization by the mouse embryo

Bisogno S , Arena R , Fic K , Gąsior Ł , Ptak GE

High numbers of lipid droplets (LDs) in mammalian eggs are stored and maintained throughout embryo development without marked signs of their utilization. It was previously demonstrated in large domestic mammals that removing lipids from the zygote does not influence post-implantation development in terms of the rate of delivered offspring. Previously studied pig and cow eggs contain considerable amounts of LDs, while mice have a very low level of ooplasmic lipids, which allows...

bp0010ised10 | (1) | ISED2019

Transcriptome analysis of blastocysts acquiring implantation competency in mice

Kong S , Wang H

Blastocyst activation, referring to the blastocyst acquiring the implantation competency, is the determining factor for implantation into the receptive uterus. It involves the process of embryonic cell differentiation to contact and initiate dialog with the uterine cells. Before the application of -omics approaches to this biological event, only the cellular morphological changes and a small number of molecules were known to regulate this process. This review aims to discuss t...

bp0010ised11 | (1) | ISED2019

Autophagy and multivesicular body formation in blastocysts during the experimental diapause in mice

Lim HJ , Shin H , Hyun Jun J , Song H

In experimentally induced diapause model in mice, blastocysts remain dormant for an extended period but resume implantation competency upon estrogen injection. The underlying mechanism by which extended longevity of dormant blastocysts is maintained is unclear. We have previously shown that dormant blastocysts, during experimentally induced diapause, exhibit heightened autophagic activation. Activation of autophagy appears to be a crucial adaptive response for survival in the ...

bp0010ised12 | (1) | ISED2019

Gliding into diapause: early embryo development in roe deer (Capreolus capreolus)

Drews B , Ulbrich SE , Rudolf Vegas A , Jewgenow K , Zahmel J , Roellig K , Ortmann S , Hildebrandt TB , Goeritz F

During mammalian diapause, the embryo is arrested at the blastocyst stage. In diapausing carnivores and marsupials, growth arrest of the blastocyst is coincident with a quiescent corpus luteum (CL). In the roe deer, progesterone is produced throughout diapause and the blastocyst exhibits very slow growth. To date, early roe deer embryos of known age have not been described. Therefore, it is not known at which stage and at what pace the roe deer embryo enters diapause. To colle...

bp0010ised13 | (1) | ISED2019

Trophectoderm and embryoblast proliferate at slow pace in the course of embryonic diapause in the roe deer (Capreolus capreolus)

Rüegg AB , Bernal S , Moser FN , Rutzen I , Ulbrich SE

The European roe deer (Capreolus capreolus) was the first mammal in which embryonic diapause has been described. While diapause is characterized by a complete developmental arrest in some species, roe deer blastocysts show a very slow, yet continuous growth. To date, it is neither known whether this growth is accompanied by developmental progression nor whether it is uniform in both, the trophectoderm (TE) and the inner cell mass (ICM). We collected roe deer blastocysts during...

bp0010ised14 | (1) | ISED2019

Delayed implantation combined with precocious sexual maturation in female offspring: a story of the stoat

Amstislavsky S , Brusentsev E , Kizilova E

The objective of this study was to investigate the precocious sexual maturation in stoat females. We confirmed oestrus and successful mating in newborn stoats; and documented ovulation, preimplantation embryo development, embryonic diapause and implantation during first nine months of life. A total of 100 embryos at different stages of development were flushed from the oviducts and uterine horns obtained from female stoats (Mustela erminea) between day 26 and day 251 ...

bp0010ised15 | (1) | ISED2019

Embryonic diapause modulates ovarian follicular dynamics and oocyte quality in the European roe deer (Capreolus capreolus)

Bernal-Ulloa SM , Bick JT , van der Weijden VA , Rüegg AB , Drews B , Kowalewski MP , Bauersachs S , Ulbrich SE

The phenomenon of embryonic diapause has been reported to possibly affect ovarian follicular development. The aim of the current study was to identify the impact of the embryonic diapause period on follicle number and oocyte quality in the European roe deer (Capreolus capreolus). In this species, the embryo undergoes five months of developmental delay. We have determined the antral follicle count (AFC), oocyte morphological quality, oocyte meiotic resumption capacity ...

bp0010ised16 | (1) | ISED2019

New directions to understand and learn from embryonic diapause in mammals

Comizzoli P

Embryonic diapause is a key strategy to extend pregnancy until conditions are ideal for birth and postnatal survival. There is still a lot to discover about this unique phenomenon observed in more than 130 mammalian species. The present review aims at complementing existing research efforts by (1) identifying new directions for a better understanding of embryonic diapause in mammals and (2) considering this complex mechanism as a source of inspiration for other areas in cellul...

bp0010ised17 | (1) | ISED2019

Summary of the Third International Symposium on Embryonic Diapause

Renfree MB , Murphy BD

Abstract unavailable© Third International Symposium on Embryonic Diapause...