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Bioscientifica Proceedings (2020) 19 CPRCPR4 | DOI: 10.1530/biosciprocs.19.0004

1 NARO Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0901, Japan; 2 National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan; 3 NARO Institute of Animal Health, Tsukuba, Ibaraki, Japan

Cryopreservation of female germplasm has basic importance in preservation and distribution of genetic lines in farm animals. Although vitrification technology has been applied for the cryopreservation of porcine oocytes and ovarian tissues, reduced developmental competence of preserved oocytes and the lack of offspring produced from them underlines the importance for further developments in cryopreservation protocols for this purpose. This review discusses the problems of female germplasm cryopreservation in pigs and the possible strategies to overcome them and gives an update on the present status of cryopreservation of porcine oocytes and ovarian tissues.

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