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Bioscientifica Proceedings (2020) 18 CPRCPR1 | DOI: 10.1530/biosciprocs.18.0001

1Division of Reproduction, Faculty of Veterinary Medicine & Animal Science (FVMAS), Swedish University of Agricultural Sciences (SLU), POB 7054, SE-75007 Uppsala, Sweden; 2Center for Andrology & Sexual Medicine, Department of Medicine, Karolinska University Hospital/Huddinge, SE-14186 Stockholm, Sweden; 3Quality Genetics, Raby 2004, SE-24292 Hörby, Sweden; 4Department of Anatomy, Physiology & Biochemistry, FVMAS, SLU, POB 7011, SE-75007 Uppsala, Sweden; 5Laboratory of Structural Proteomics, Institute of Biomedicine of Valencia, CSIC, Jaime Roig 11, 46010 Valencia, Spain; 6Section of Reproduction & Obstetrics, Department of Medicine, Faculty of Veterinary Medicine, Avd de la Universidad sin, 10071 Cáceres, Spain; 7Department of Medicine & Animal Surgery, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain; acurrent address: Dept of Animal Sciences, Faculty of Veterinary Sciences, University of Concepción, Av. Vicente Mende 595, Chilláan, Chile

During ejaculation in the boar, sperm cohorts emitted in epididymal cauda fluid are sequentially exposed and resuspended in different mixtures of accessory sex gland secretion. This paper reviews the relevance of such unevenly composed fractions of seminal plasma (SP) in vivo on sperm transport and sperm function and how this knowledge could benefit boar semen processing for artificial insemination (Al). The firstly ejaculated spermatozoa (first 10 ml of the sperm-rich fraction, SRF [Pl]) remain mainly exposed to epididymal cauda fluid and its specific proteins i.e. various lipocalins, including the fertility-related prostaglandin D synthase; than to prostatic and initial vesicular gland secretions. Plspermatozoa are hence exposed to less bicarbonate, zinc or fructose and mainly to PSP-I spermadhesin; than if they were in the rest of the SRF and the post-SRF (P2). Since the Pl-SP is less destabilizing for sperm membrane and chromatin, Pl-spermatozoa sustain most in vitro procedures, including cryopreservation, the best. Moreover, ejaculated firstly, the Pl-spermatozoa seem also those deposited by the boar as a vanguard cohort, thus becoming overrepresented in the oviductal sperm reservoir (SR). This vanguard SR-entry occurs before the endometrial signalling of SP components (as PSP-I/PSP-11 and cytokines) causes a massive influx of the innate defensive PMNs to cleanse the uterus from eventual pathogens, superfluous spermatozoa and the allogeneic SP. The SP also conditions the mucosal immunity of the female genital tract, to tolerate the SR-spermatozoa and the semi-allogeneic conceptus. These in vivo gathered data can be extrapolated into procedures for handling boar spermatozoa in vitro for Al and other biotechnologies, including simplified cryopreservation.

© 2009 Society for Reproduction and Fertility

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